| Summary: | The toughest organisms on Earth, called extremophiles, can survive extreme conditions like extreme dryness (desiccation), extreme cold, space vacuum, acid, or even high-level radiation. So far, the toughest of all seems to be the bacterium Deinococcus radioduransâ??able to survive doses of radiation a thousand times greater than those fatal to humans. But to this date, scientists remained puzzled by how radio-resistance could have evolved in several organisms on our planet, naturally protected from solar radiation by its magnetic field. While some scientists suggest that radio-resistance in extremophile organisms could have evolved along with other kinds of resistance, such as resistance to desiccation, a question remained: which genes are specifically involved in radio-resistance? To address this question, the team of Dr. Cox, at the University of Wisconsin-Madison, decided to "let the cells tell them". The researchers started with the naturally non-resistant bacteria, E. coli, and ex In their first study, the team of Dr. Cox started by exposing E. coli to 50 rounds of ionization (Bruckbauer et al 2019b). After about 10 rounds, some radio-resistant populations emerged, and after 50, the study of their genetic profile highlighted three mutations responsible for radio-resistanceâ??all in genes linked to DNA repair mechanisms. Here, in their new study, the team exposed the bacteria to 50 more rounds of radiation exposure and selection. The results published in Frontiers in Microbiology show that the populations of radioresistant E. coli, continued to evolve and sub-populations emerged. Surprisingly, while radio-resistance induced by the first series of ionization could mainly be associated with three mutations, the second induced hundreds of mutations including large deletions and duplications of several genes. "The four populations we are evolving in this new trial have now achieved levels of radio-resistance that are approaching the levels seen... |